Validating IAAO for Muscle Outcomes
Protein Metabolism
De qué trata este estudio
El registro no identifica actualmente este estudio como reclutando. Consulta su estado antes de planificar una evaluación.
- Tipo de estudio
- De intervención
- Fase
- No aplica
- Rango de edad
- 18 años - 35 años
- Sexo admitido
- Todos los sexos
- Voluntarios sanos
- Aceptados
- Participantes
- 8 Estimado
Estos son puntos de partida, no una decisión de elegibilidad. El equipo debe revisar todos los requisitos.
Las explicaciones y la navegación están en español. Los textos proporcionados por el registro, incluidos títulos, resúmenes y criterios, se conservan en su idioma original. Puedes pedir orientación en español a WCR.
Acerca de la investigación
Consuming dietary protein stimulates whole-body and muscle protein synthesis, the latter of which is typically measured using invasive primed constant infusions of stable isotopes with concurrent muscle biopsies. Alternative non-invasive methodologies have been developed (namely the indicator amino acid oxidation (IAAO) technique) to estimate the impact of protein ingestion on whole-body protein synthesis as a proxy for determining dietary protein requirements. Given that the IAAO technique is based on principles of protein metabolism which occur in the liver, it is unclear how representative the IAAO outcomes of whole-body protein synthesis is to skeletal muscle protein synthesis. Validation of the IAAO technique against gold-standard, biopsy-derived measures of muscle metabolism (i.e., muscle protein synthesis) would assist in mitigating the invasiveness of muscle physiology and nutrition research.
¿Quién podría participar?
La lista siguiente contiene el texto del patrocinador. Puede incluir condiciones, tratamientos previos, resultados de pruebas y otros requisitos. Aparecer en tu búsqueda no significa que califiques.
Inclusion Criteria:
- Participants will be aged 18-35 years old
- Participants will have a BMI within the normal range (i.e., 18.5-24.9) and waist-to-hip circumference ratio of \<0.95 for males and \<0.8 for circumference for females to ensure homogeneity of the sample population
- Participants are willing to abide by the compliance rules of this study (e.g., abstain from extraneous physical activity 48h prior to session 4
- Self-reported regular menstrual cycle (25-35d) within the last 3 months (female participants)
- Physical activity score of ≥24 units as measured by the Godin leisure time exercise questionnaire
Exclusion Criteria:
- Inability to adhere to any of the compliance rules judged by principal investigator
- Self-reported regular tobacco or illicit drug use (e.g., growth hormone or testosterone)
- Current use of hormonal contraceptives
- Individuals with a history of allergy to local anesthetics (e.g., lidocaine)
Tratamiento, grupos y participación
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- Asignación
- No aplica
- Enmascaramiento
- Sin enmascaramiento
Protein + Stable Isotope Tracer Beverage
Participants will consume 12 half-hourly (6 hours) isoenergetic, isonitrogenous beverages containing 0.9g/kg fat-free mass/day protein. Drinks will be enriched with stable isotopes \[2H5\]Phenylalanine and \[1-13C\]Phenylalanine, which will respectively allow for determination of muscle protein synthesis and whole-body protein synthesis over the subsequent 6 hours of feeding.
Dietary Supplement: Protein + Stable Isotope Tracer Beverage
Protein + Stable Isotope Tracer Beverage DIETARY_SUPPLEMENT
Participants will consume 12 half-hourly (6 hours) isoenergetic, isonitrogenous beverages containing 0.9g/kg fat-free mass/day protein. Drinks will be enriched with stable isotopes \[2H5\]Phenylalanine and \[1-13C\]Phenylalanine, which will respectively allow for determination of muscle protein synthesis and whole-body protein synthesis over the subsequent 6 hours of feeding
Qué evalúan los investigadores
Estas medidas indican qué se estudia. No prueban que un tratamiento funcione.
Medidas principales
Phenylalanine excretion (umol/kg/h)
Plazo: 6.5 hours
Rate of whole-body phenylalanine excretion following orally ingested amino acid tracer \[1-13C\]Phenylalanine determined from the product of breath 13CO2 enrichment and carbon dioxide production rate (VCO2).
Phenylalanine oxidation (umol/kg/h)
Plazo: 6.5 hours
Rate of whole-body phenylalanine oxidation following orally ingested amino acid tracer \[1-13C\]Phenylalanine determined from the correction of phenylalanine excretion by the phenylalanine precursor enrichment via urine enrichment.
Myofibrillar protein synthesis (%/hr)
Plazo: 6.5 hours
Rate of myofibrillar fractional synthesis is determined by rate of incorporation of orally ingested tracer \[2H5\]Phenylalanine into myofibrillar proteins (isolated from skeletal muscle biopsies) and the enrichment of \[2H5\]Phenylalanine in plasma collected over the 6 hours protein consumption period. Enrichment will be quantified via tandem LC-MS/MS to derive a rate of incorporation over the 6-hour period.
Sarcoplasmic protein synthesis (%/hr)
Plazo: 6.5 hours
Rate of sarcoplasmic fractional synthesis is determined by rate incorporation of orally ingested tracer \[2H5\]Phenylalanine into sarcoplasmic (ie., soluble) proteins (isolated from skeletal muscle biopsies) and the enrichment of \[2H5\]Phenylalanine in plasma collected over the 6 hours protein consumption period. Enrichment will be quantified via tandem LC-MS/MS to derive a rate of incorporation over the 6-hour period.
Revisa las tablas completas y sus limitaciones en el registro oficial.
Abrir resultados oficiales ↗Centros de investigación (1)
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1 centros
Kinesiology & Physical EducationToronto, Ontario, CanadaEstado desconocido
Equipo y fechas
- Patrocinador principal
- University of Toronto
- Inicio del estudio
- 1 dic 2025 Real
- Finalización primaria
- 17 abr 2026 Real
- Finalización del estudio
- 20 dic 2026 Estimado
- Primera publicación
- 5 ene 2026 Real
- Última actualización
- 9 nov 2026 Real
Contactos oficiales del estudio
No hay un contacto central indicado. Revisa los centros y el registro oficial.
Título oficial del estudio
Validating the Indicator Amino Acid Oxidation Technique for Muscle Outcomes
Consulta a Wellness Clinical Research
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Fuente: API v2 de ClinicalTrials.gov. Patrocinadores e investigadores envían la información. La NLM no aprueba la seguridad ni la ciencia de todos los estudios registrados. Aviso del registro.
Consultado: 12 nov 2026 · Última actualización del registro: 9 nov 2026